Ukusetshenziswa kobuchwepheshe bokupholisa be-thermoelectric kumathuluzi e-PCR

Ukusetshenziswa kobuchwepheshe bokupholisa be-thermoelectric kumathuluzi e-PCR

Ukusetshenziswa kobuchwepheshe bokupholisa be-thermoelectric kumathuluzi e-PCR kulele ekulawuleni izinga lokushisa. Inzuzo yayo eyinhloko ikhono lokulawula izinga lokushisa elisheshayo nelinembile, eliqinisekisa izinga lempumelelo lokuhlolwa kokukhulisa i-DNA.

Izimo zohlelo lokusebenza ezibalulekile

1. Ukulawula izinga lokushisa okunembile

Ithuluzi le-PCR lidinga ukujikeleza ezigabeni ezintathu: ukushintshwa kwezinga lokushisa eliphezulu (90-95℃), ukwehlisa izinga lokushisa eliphansi (55-65℃), kanye nokunwetshwa kwezinga lokushisa elilungile (70-75℃). Izindlela zesiqandisi zendabuko zinzima ukuhlangabezana nemfuneko yokunemba engu-±0.1℃. Ukupholisa kwe-Thermoelectric, ubuchwepheshe bokupholisa be-peltier bufinyelela ukulawulwa kwezinga lokushisa kwe-millisecond ngomphumela we-Peltier, ukugwema ukwehluleka kokukhulisa okubangelwa umehluko wezinga lokushisa elingu-2℃.

2. Ukupholisa okusheshayo nokushisisa

Amamojula okupholisa e-Thermoelectric, amamojula we-thermoelectric, amadivaysi e-peltier, amamojula we-peltier angakwazi ukufeza izinga lokupholisa lika-3 kuya ku-5 degrees Celsius ngomzuzwana, afinyeze kakhulu umjikelezo wokuhlola uma kuqhathaniswa nama-degree Celsius angu-2 ngomzuzwana we-compressor evamile. Isibonelo, insimbi ye-PCR engu-96-well isebenzisa ubuchwepheshe bokulawula izinga lokushisa lendawo ukuze kuqinisekiswe amazinga okushisa angaguquki kuzo zonke izindawo zemithombo futhi igweme umehluko wezinga lokushisa ongu-2℃ obangelwa imithelela enqenqemeni.

3. Thuthukisa ukwethembeka kwemishini

Amamojula okupholisa ugesi we-thermoelectric, amamojula we-peltier, ama-peltier lements, amamojula e-TEC e-Beijing Huimao Cooling Equipment Co., Ltd. asephenduke izingxenye eziyinhloko zokulawula izinga lokushisa lamathuluzi e-PCR ngenxa yokuthembeka kwawo okuphezulu. Usayizi wayo omncane nezici ezingenawo msindo ziyenza ifanelekele izidingo ezinembayo zemishini yezokwelapha.

Izimo zohlelo lokusebenza ezijwayelekile

I-96-well fluorescence quantitative PCR detector: Ihlanganiswe ne-thermoelectric cooling module, imojula ye-TEC, idivayisi ye-peltier, amamojula we-peltier inika amandla ukulawulwa okunembile kwezinga lokushisa kwamasampuli aphezulu futhi isetshenziswa kabanzi emikhakheni efana nokuhlaziywa kwe-gene expression kanye nokutholwa kwe-pathogen.

Iziqandisi eziphathwayo zezokwelapha: ukupholisa i-thermoelectric, iziqandisi eziphathwayo zokupholisa ze-peltier ezisetshenziselwa ukugcina imikhiqizo efana nemithi yokugoma nemithi edinga indawo enezinga lokushisa eliphansi, iqinisekisa ukuzinza kwezinga lokushisa ngesikhathi sokuthutha.

Imishini yokwelapha nge-laser:

Amamojula wokupholisa we-Thermoelectric, izakhi ze-peltier, amamojula we-thermoelectric apholisa i-laser emitter ukunciphisa ingozi yokushiswa kwesikhumba futhi kuthuthukise ukuphepha kokwelashwa.

Imifanekiso ye-TEC1-39109T200

Izinga lokushisa ohlangothini olushisayo ngu-30 C,

Isilinganiso: 9A

Ubukhulu: 46V

Ubukhulu: 246.3W

I-ACR: 4±0.1Ω (Ta= 23 C)

Ubukhulu be-Delta T: 67 -69C

Usayizi: 55x55x3.5-3.6mm

Imifanekiso ye-TES1-15809T200

Izinga lokushisa ohlangothini olushisayo: 30 C,

Isilinganiso: 9.2A,

Ubukhulu: 18.6V

Ubukhulu: 99.5 W

Ubukhulu be-Delta T: 67 C

I-ACR:1.7 ±15% Ω (1.53 kuya ku-1.87 Ohm)

Usayizi: 77×16.8×2.8mm

Ucingo: I-silicone wire engu-18 AWG noma i-Sn-plated elinganayo ebusweni, izinga lokushisa eliphezulu Ukumelana no-200 ℃